Zeng L. Xiao Y. Li X. Zhou Y. Fan Y.
A cell line, designated PF-Fin, had been established from the fin of paddlefish, Polyodon spathula Walbaum and sub-cultured over 70 times. Characterization of the cell line included determination of optimal growth kinetics, plating efficiency, karyotyping, and 28S ribosome RNA genotyping, etc. The primary culture of this cell was generated by the tissue explantation technique using the M199 medium supplemented with 30% fetal bovine serum, epidermal/fibroblast growth factors and penicillin/streptomycin/amphotericin B solution. Subculture of the cell was conducted in M199 medium with 10% fetal bovine serum. The cell grew between 20 – 30 C° and the optimal growth temperature was 25 C°. The cell monolayer consisted of fibroblast - like cells and the plating efficiency at 49th passage was about 13.75%. Following cryopreservation in liquid nitrogen, thawed cells exhibited a viability of >85% after 12 months storage period. Chromosome typing of this cell line revealed the diploid chromosome number was 2n = 120 at its 5th passage, and at its 56th passage the modal chromosome number is 2n =90. Polymerase chain reaction amplification of partial 28S ribosome RNA and sequencing analysis indicated 98% identity of the sequence found in GenBank from Acipenser transmontanus , Richardson, confirming that the cell line was of sturgeon origin.
